Two novel oligopeptide transporter cDNA clones, CPTA and CPTB, were identified by screening a Caenorhabditis elegans cDNA library using homology hybridization. The transporter proteins deduced from the cDNAs possess multiple transmembrane domains and reveal a moderate similarity to their mammalian counterparts in amino acid sequences. CPTA and CPTB, when expressed in Xenopus laevis oocytes and studied by both radiotracer flux and microelectrode voltage-clamp protocol, displayed a saturable electrogenic transport activity driven by a proton gradient with an overlapping broad spectrum of substrate specificity. Both transporters recognize di-, tri- and tetra-peptides including phenylalanylmethionylarginylphenylalaninamide (FMRFamide) and N-acetylaspartylglutamate, members of a large neuropeptide family commonly found throughout the animal kingdom. Kinetic analysis, however, revealed that CPTA and CPTB differed in their affinity for the peptide substrates, the former being a high-affinity type and the latter a low-affinity type. CPTA and CPTB are encoded by two distinct genes localized on separate chromosomes and are expressed during the whole life span of the organism.
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June 1998
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Research Article|
June 01 1998
Two oligopeptide transporters from Caenorhabditis elegans:molecular cloning and functional expression
You-Jun FEI;
You-Jun FEI
1
1Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA 30912, U.S.A.
1To whom correspondence should be addressed (email: yjfei@mail.mcg.edu).
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Takuya FUJITA;
Takuya FUJITA
1Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA 30912, U.S.A.
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David F. LAPP;
David F. LAPP
1Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA 30912, U.S.A.
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Vadivel GANAPATHY;
Vadivel GANAPATHY
1Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA 30912, U.S.A.
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Frederick H. LEIBACH
Frederick H. LEIBACH
1Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA 30912, U.S.A.
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Publisher: Portland Press Ltd
Received:
November 25 1997
Revision Received:
February 16 1998
Accepted:
February 27 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 332 (2): 565–572.
Article history
Received:
November 25 1997
Revision Received:
February 16 1998
Accepted:
February 27 1998
Citation
You-Jun FEI, Takuya FUJITA, David F. LAPP, Vadivel GANAPATHY, Frederick H. LEIBACH; Two oligopeptide transporters from Caenorhabditis elegans:molecular cloning and functional expression. Biochem J 1 June 1998; 332 (2): 565–572. doi: https://doi.org/10.1042/bj3320565
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