Studies of inositol lipid-specific phospholipase C (PLC) have elucidated the main regulatory pathways for PLCβ and PLCγ but the regulation of PLCΔ isoenzymes still remains obscure. Here we demonstrate that an increase in Ca2+ ion concentration within the physiological range (0.1-10 μM) is sufficient to stimulate PLCΔ1, but not PLCγ1 and PLCβ1, to hydrolyse cellular inositol lipids present in permeabilized cells. The activity of PLCΔ1 is further enhanced in the presence of phosphatidylinositol transfer protein (PI-TP). Both full activation by Ca2+ ions and stimulation in the presence of PI-TP require an intact PH domain involved in the membrane attachment of PLCδ1. The physiological implication of this study is that PLCΔ1 could correspond to a previously uncharacterized PLC responsible for Ca2+ ion-stimulated inositol lipid hydrolysis observed in many cellular systems.
Skip Nav Destination
Article navigation
October 1997
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Research Article|
October 15 1997
Regulation of inositol lipid-specific phospholipase Cδ by changes in Ca2+ ion concentrations
Victoria ALLEN;
Victoria ALLEN
*CRC Centre for Cell and Molecular Biology, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, U.K.
Search for other works by this author on:
Philip SWIGART;
Philip SWIGART
†Department of Physiology, Rockefeller Building, University College London, University Street, London W1P 8BT, U.K.
Search for other works by this author on:
Robert CHEUNG;
Robert CHEUNG
*CRC Centre for Cell and Molecular Biology, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, U.K.
Search for other works by this author on:
Shamshad COCKCROFT;
Shamshad COCKCROFT
1
†Department of Physiology, Rockefeller Building, University College London, University Street, London W1P 8BT, U.K.
1Correspondence could be addressed to either of these authors.
Search for other works by this author on:
Matilda KATAN
Matilda KATAN
1
*CRC Centre for Cell and Molecular Biology, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, U.K.
1Correspondence could be addressed to either of these authors.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
April 02 1997
Revision Received:
June 23 1997
Accepted:
June 27 1997
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1997
1997
Biochem J (1997) 327 (2): 545–552.
Article history
Received:
April 02 1997
Revision Received:
June 23 1997
Accepted:
June 27 1997
Citation
Victoria ALLEN, Philip SWIGART, Robert CHEUNG, Shamshad COCKCROFT, Matilda KATAN; Regulation of inositol lipid-specific phospholipase Cδ by changes in Ca2+ ion concentrations. Biochem J 15 October 1997; 327 (2): 545–552. doi: https://doi.org/10.1042/bj3270545
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.