Human α2-antiplasmin (α2AP) is a serpin involved in the regulation of blood coagulation. Most serpins, unlike smaller serine proteinase inhibitors, do not contain disulphide bridges. α2AP is an exception from this generalization and has previously been shown to contain four Cys residues organized into two disulphide bridges [Lijnen, Holmes, van Hoef, Wiman, Rodriguez and Collen (1987) Eur. J. Biochem. 166, 565–574]. However, we found that α2AP incorporates iodo[14C]acetic acid, suggesting that the protein contains reactive thiol groups. This observation prompted a re-examination of the state of the thiol groups, which revealed (i) a disulphide bridge between Cys43 and Cys116, (ii) that Cys76 is bound to a cysteinyl-glycine dipeptide, and (iii) and Cys125 exists as either a free thiol or in a mixed disulphide with another Cys residue. The disulphide identified between Cys43 and Cys116 appears to be conserved in orthologous proteins since the homologous Cys residues form disulphide bonds in bovine and possibly mouse α2AP. The conservation of this disulphide bridge suggests that it is important for functional aspects of α2AP. However, the structural and functional analysis described in this study does not support this conclusion.
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May 1997
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Research Article|
May 01 1997
Assignment of a single disulphide bridge in human α2-antiplasmin: implications for the structural and functional properties
Søren CHRISTENSEN;
Søren CHRISTENSEN
1Duke University Medical Center, Department of Pathology, P.O. Box 3712, Durham, NC 27710, U.S.A.
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Zuzana VALNICKOVA;
Zuzana VALNICKOVA
1Duke University Medical Center, Department of Pathology, P.O. Box 3712, Durham, NC 27710, U.S.A.
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Ida B. THØGERSEN;
Ida B. THØGERSEN
1Duke University Medical Center, Department of Pathology, P.O. Box 3712, Durham, NC 27710, U.S.A.
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Eva H. N. OLSEN;
Eva H. N. OLSEN
1Duke University Medical Center, Department of Pathology, P.O. Box 3712, Durham, NC 27710, U.S.A.
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Jan J. ENGHILD
Jan J. ENGHILD
*
1Duke University Medical Center, Department of Pathology, P.O. Box 3712, Durham, NC 27710, U.S.A.
*To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
July 22 1996
Revision Received:
December 09 1996
Accepted:
December 24 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1997
1997
Biochem J (1997) 323 (3): 847–852.
Article history
Received:
July 22 1996
Revision Received:
December 09 1996
Accepted:
December 24 1996
Citation
Søren CHRISTENSEN, Zuzana VALNICKOVA, Ida B. THØGERSEN, Eva H. N. OLSEN, Jan J. ENGHILD; Assignment of a single disulphide bridge in human α2-antiplasmin: implications for the structural and functional properties. Biochem J 1 May 1997; 323 (3): 847–852. doi: https://doi.org/10.1042/bj3230847
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