In mouse NIH 3T3 cells, the mitogens bombesin and thrombin induced Ca2+ release from intracellular stores. Ca2+ release induced by bombesin was inhibited by the Ca2+-ATPase inhibitor thapsigargin, while Ca2+ release induced by thrombin was unaffected by this agent. The Ca2+-release response to bombesin was not affected by pertussis toxin, but the response to thrombin was abolished by the toxin. Stable transfectants overexpressing the G-protein subunit type αq showed an accentuated response to bombesin, indicating that the bombesin receptor was coupled to a Gq-like G-protein. Together, these results show that the two mitogenic receptors are coupled to distinct G-proteins that affect functionally different pools of Ca2+. Organization of signalling pathways in this manner may allow cells to differentially encode information from different signals.
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November 1996
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Research Article|
November 15 1996
Bombesin and thrombin affect discrete pools of intracellular calcium through different G-proteins
Jin-Lin WANG;
Jin-Lin WANG
*Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, U.S.A.
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Somasundaram KALYANARAMAN;
Somasundaram KALYANARAMAN
*Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, U.S.A.
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Michael DE VIVO;
Michael DE VIVO
‡Department of Pharmacology, Mount Sinai School of Medicine, City University of New York, NY 10029, U.S.A.
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Narasimhan GAUTAM
Narasimhan GAUTAM
§
*Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, U.S.A.
†Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, U.S.A.
§To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
April 15 1996
Revision Received:
July 12 1996
Accepted:
July 19 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 320 (1): 87–91.
Article history
Received:
April 15 1996
Revision Received:
July 12 1996
Accepted:
July 19 1996
Citation
Jin-Lin WANG, Somasundaram KALYANARAMAN, Michael DE VIVO, Narasimhan GAUTAM; Bombesin and thrombin affect discrete pools of intracellular calcium through different G-proteins. Biochem J 15 November 1996; 320 (1): 87–91. doi: https://doi.org/10.1042/bj3200087
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