Interleukin-11 (IL-11) is a polyfunctional cytokine whose biological actions require a specific IL-11 receptor (IL-11R) and the transmembrane transducer gp130. Here we report the production of a soluble form of the murine IL-11R and demonstrate that it interacts with IL-11 ligand with high affinity. The affinity of IL-11 alone for gp130 is below the level of detection, but a complex of IL-11 and soluble IL-11R interacts with gp130 with high affinity. The addition of soluble IL-11R potentiates the effects of exogenous IL-11 in cells that are normally responsive to IL-11. A biological response to IL-11 can be reconstituted in BAF cells transfected with gp130 by addition of IL-11 and soluble IL-11R. These findings show that the cytoplasmic domain of the IL-11R is not required for the biological effects of IL-11 and that a complex of IL-11 and IL-11R mediates signalling by association with gp130.
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September 1996
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Research Article|
September 01 1996
Mediation of interleukin-11-dependent biological responses by a soluble form of the interleukin-11 receptor
Julia KAROW;
Julia KAROW
*CRC Growth Factor Group, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K.
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Keith R. HUDSON;
Keith R. HUDSON
*CRC Growth Factor Group, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K.
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Mark A. HALL;
Mark A. HALL
*CRC Growth Factor Group, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K.
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Ann B. VERNALLIS;
Ann B. VERNALLIS
*CRC Growth Factor Group, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K.
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Jacky A. TAYLOR;
Jacky A. TAYLOR
*CRC Growth Factor Group, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K.
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Achim GOSSLER;
Achim GOSSLER
†The Jackson Laboratory, 600 Main St., Bar Harbor, ME 04609-1500, U.S.A.
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John K. HEATH
John K. HEATH
§
*CRC Growth Factor Group, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K.
‡Department of Biochemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, U.K.
§To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
November 07 1995
Revision Received:
April 24 1996
Accepted:
May 30 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 318 (2): 489–495.
Article history
Received:
November 07 1995
Revision Received:
April 24 1996
Accepted:
May 30 1996
Citation
Julia KAROW, Keith R. HUDSON, Mark A. HALL, Ann B. VERNALLIS, Jacky A. TAYLOR, Achim GOSSLER, John K. HEATH; Mediation of interleukin-11-dependent biological responses by a soluble form of the interleukin-11 receptor. Biochem J 1 September 1996; 318 (2): 489–495. doi: https://doi.org/10.1042/bj3180489
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