Expression of the muscle-specific 2a isoform of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2) requires activation of an otherwise inefficient splice process at the 3´-end of the primary gene transcript. We provide evidence that SERCA2 splicing is a specifically regulated process, rather than the result of an increase in general splice efficiency or a decrease in polyadenylation efficiency at the 5´-most polyadenylation site. This is indicated by the fact that changes in general splice and polyadenylation efficiency, as observed during B-cell maturation, did not affect SERCA2 splicing. Furthermore, expression and overexpression studies did not support the hypothesis that changes in the level of the alternative splice factor ASF/SF2 or other arginine and serine rich proteins are sufficient to obtain the regulation of muscle- and neuronal-specific splicing.
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August 1996
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Research Article|
August 01 1996
Alternative processing of the sarco/endoplasmic reticulum Ca2+-ATPase transcripts during muscle differentiation is a specifically regulated process
Ludo VAN DEN BOSCH;
*Laboratory of Physiology, Campus Gasthuisberg, University of Leuven, KULeuven, B-3000 Leuven, Belgium
¶To whom correspondence should be addressed.
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Luc MERTENS;
Luc MERTENS
§
*Laboratory of Physiology, Campus Gasthuisberg, University of Leuven, KULeuven, B-3000 Leuven, Belgium
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Yvon CAVALOC;
Yvon CAVALOC
†Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
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Martha PETERSON;
Martha PETERSON
‡Department of Pathology and Laboratory Medicine, University of Kentucky, College of Medicine, Lexington, KY, U.S.A.
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Frank WUYTACK;
Frank WUYTACK
*Laboratory of Physiology, Campus Gasthuisberg, University of Leuven, KULeuven, B-3000 Leuven, Belgium
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Jan EGGERMONT
Jan EGGERMONT
*Laboratory of Physiology, Campus Gasthuisberg, University of Leuven, KULeuven, B-3000 Leuven, Belgium
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Publisher: Portland Press Ltd
Received:
May 13 1996
Revision Received:
May 31 1996
Accepted:
June 03 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 317 (3): 647–651.
Article history
Received:
May 13 1996
Revision Received:
May 31 1996
Accepted:
June 03 1996
Citation
Ludo VAN DEN BOSCH, Luc MERTENS, Yvon CAVALOC, Martha PETERSON, Frank WUYTACK, Jan EGGERMONT; Alternative processing of the sarco/endoplasmic reticulum Ca2+-ATPase transcripts during muscle differentiation is a specifically regulated process. Biochem J 1 August 1996; 317 (3): 647–651. doi: https://doi.org/10.1042/bj3170647
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