Ornithine decarboxylase (ODC) is degraded in an ATP-dependent manner in vitro by the 26 S proteasome in the presence of antizyme, an ODC destabilizing protein induced by polyamines. In the present study we examined whether the proteasome catalyses ODC degradation in living mammalian cells. Lactacystin, the most selective proteasome inhibitor, strongly inhibited the degradation of ODC that had been induced in hepatoma tissue-culture (HTC) cells by refeeding with fresh medium. Furthermore the inhibitor inhibited the rapid degradation of ODC that had been induced by hypotonic shock. Interestingly, hypertonic shock was found to increase the proportion of ODC present as a complex with antizyme (the ratio of ODC–antizyme complex to total ODC). Cycloheximide, which partly inhibits rapid ODC degradation caused by hypertonic shock, also partly inhibited the increase in the ratio of ODC–antizyme complex to total ODC. These results suggest that a common ODC degradation pathway, namely the antizyme-dependent and 26 S proteasome-catalysed ODC degradation pathway, is also operating in intact cells for osmoregulated ODC degradation.
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July 1996
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Research Article|
July 01 1996
Proteasome pathway operates for the degradation of ornithine decarboxylase in intact cells
Yasuko MURAKAMI;
Yasuko MURAKAMI
§
*Department of Biochemistry 2, The Jikei University School of Medicine, Minato-ku, Tokyo 105, Japan
§To whom correspondence should be addressed.
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Nobuyuki TANAHASHI;
Nobuyuki TANAHASHI
†Institute for Enzyme Research, The University of Tokushima, Kuramoto-cho, Tokushima 770, Japan
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Keiji TANAKA;
Keiji TANAKA
†Institute for Enzyme Research, The University of Tokushima, Kuramoto-cho, Tokushima 770, Japan
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Satoshi ŌMURA;
Satoshi ŌMURA
‡The Kitasato Institute, 9-1 Shirokane 5-chome, Minato-ku, Tokyo 108, Japan
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Shin-ichi HAYASHI
Shin-ichi HAYASHI
*Department of Biochemistry 2, The Jikei University School of Medicine, Minato-ku, Tokyo 105, Japan
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Publisher: Portland Press Ltd
Received:
November 20 1995
Revision Received:
February 12 1996
Accepted:
February 27 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 317 (1): 77–80.
Article history
Received:
November 20 1995
Revision Received:
February 12 1996
Accepted:
February 27 1996
Citation
Yasuko MURAKAMI, Nobuyuki TANAHASHI, Keiji TANAKA, Satoshi ŌMURA, Shin-ichi HAYASHI; Proteasome pathway operates for the degradation of ornithine decarboxylase in intact cells. Biochem J 1 July 1996; 317 (1): 77–80. doi: https://doi.org/10.1042/bj3170077
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