Specific binding of 125I-labelled bovine odour-binding protein (OBP) to isolated membranes from nasal mucosa was demonstrated. The interaction reached equilibrium within 30 min at 37 °C and was reversible. A Scatchard analysis of the equilibrium binding revealed a single population of binding sites, with the calculated equilibrium dissociation constant and maximum number of binding sites being 2.25±0.5 μM and 18.5±2 pmol/mg of membrane protein respectively (n = 2). Receptor activity was decreased on digestion by trypsin, proteinase K or endoglycosidase H, was heat labile and was sensitive to thiol-group-specific reagents. With the exception of rat and mouse major urinary proteins, which exhibit a high degree of structural similarity with OBP and bind similar ligands, other members of the lipocalin family, such as retinol-binding protein and β-lactoglobulin, failed to inhibit the binding of 125I-labelled OBP to its receptor. The receptor seems not to be restricted to olfactory tissues, as it was detected in a variety of other tissues. This suggests that OBP is unlikely to play a role only in olfactory signal transduction. It might have a much broader role within the body; possibilities include a role in detoxification or signalling.
Skip Nav Destination
Article navigation
July 1996
-
Cover Image
Cover Image
- PDF Icon PDF LinkTable of Contents
Research Article|
July 01 1996
Membrane receptor for odour-binding proteins
Mohamed BOUDJELAL;
Mohamed BOUDJELAL
‡
*Department of Biochemistry and Molecular Biology
Search for other works by this author on:
Asipu SIVAPRASADARAO;
Asipu SIVAPRASADARAO
§
†Department of Pharmacology, University of Leeds, Leeds LS2 9JT, U.K.
§To whom correspondence should be addressed.
Search for other works by this author on:
John B. C FINDLAY
John B. C FINDLAY
*Department of Biochemistry and Molecular Biology
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
September 25 1995
Revision Received:
March 18 1996
Accepted:
April 03 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 317 (1): 23–27.
Article history
Received:
September 25 1995
Revision Received:
March 18 1996
Accepted:
April 03 1996
Citation
Mohamed BOUDJELAL, Asipu SIVAPRASADARAO, John B. C FINDLAY; Membrane receptor for odour-binding proteins. Biochem J 1 July 1996; 317 (1): 23–27. doi: https://doi.org/10.1042/bj3170023
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.