The activation of hepatic acetyl-CoA carboxylase by Na+-co-transported amino acids such as glutamine has been attributed mainly to the stimulation of its dephosphorylation by accumulating dicarboxylic acids, e.g. glutamate. We report here on a hepatic species of protein phosphatase-2A that activates acetyl-CoA carboxylase in the presence of physiological concentrations of glutamate or Mg2+ and, under these conditions, accounts for virtually all the hepatic acetyl-CoA carboxylase phosphatase activity. Glutamate also stimulated the dephosphorylation of a synthetic pentadecapeptide encompassing the Ser-79 phosphorylation site of rat acetyl-CoA carboxylase, but did not affect the dephosphorylation of other substrates such as phosphorylase. Conversely, protamine, which stimulated the dephosphorylation of phosphorylase, inhibited the activation of acetyl-CoA carboxylase. A comparison with various species of muscle protein phosphatase-2A showed that the stimulatory effects of glutamate and Mg2+ on the acetyl-CoA carboxylase phosphatase activity are largely mediated by the regulatory A subunit. Glutamate and Mg2+ emerge from our study as novel regulators of protein phosphatase-2A when acting on acetyl-CoA carboxylase.
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Research Article|
May 15 1996
Activation of hepatic acetyl-CoA carboxylase by glutamate and Mg2+ is mediated by protein phosphatase-2A
Vinciane GAUSSIN;
Vinciane GAUSSIN
*Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, University of Louvain Medical School, B-1200 Brussels, Belgium
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Louis HUE;
Louis HUE
*Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, University of Louvain Medical School, B-1200 Brussels, Belgium
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Willy STALMANS;
Willy STALMANS
†Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
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Mathieu BOLLEN
Mathieu BOLLEN
‡
†Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
‡To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
October 12 1995
Revision Received:
January 02 1996
Accepted:
January 23 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 316 (1): 217–224.
Article history
Received:
October 12 1995
Revision Received:
January 02 1996
Accepted:
January 23 1996
Citation
Vinciane GAUSSIN, Louis HUE, Willy STALMANS, Mathieu BOLLEN; Activation of hepatic acetyl-CoA carboxylase by glutamate and Mg2+ is mediated by protein phosphatase-2A. Biochem J 15 May 1996; 316 (1): 217–224. doi: https://doi.org/10.1042/bj3160217
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