The expression of ferredoxin–NADP+ reductase (FNR) from Anabaena sp. PCC 7119 in heterocysts and vegetative cells has been quantified. Specific reductase activity in heterocysts was approximately 10 times higher than in vegetative cells, corresponding to the increased FNR protein content. This was confirmed by immunoquantification of the FNR protein from whole filaments of Anabaena sp. PCC 7120 grown in media with and without combined nitrogen. Transcription of the petH gene was markedly enhanced in the absence of combined nitrogen. This suggests that the increased RNA level is mainly responsible for the up-regulation of FNR in heterocysts. As has been observed for nif genes, iron deficiency also increased transcription of petH. Characterization of the FNR purified from isolated heterocysts showed no detectable differences from the enzyme from vegetative cells. Although nitrogen stress was a key regulatory factor, localization of the petH gene in the genomic map of Anabaena PCC 7120 showed that this gene is not physically associated with the nif cluster.
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Research Article|
May 15 1996
Expression of ferredoxin-NADP+ reductase in heterocysts from Anabaena sp
Pedro RAZQUIN;
Pedro RAZQUIN
*Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009-Zaragoza, Spain
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Maria F. FILLAT;
Maria F. FILLAT
*Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009-Zaragoza, Spain
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Stefan SCHMITZ;
Stefan SCHMITZ
†Botanisches Institut der Universität Bonn, Abteilung Molekulare Biochemie, Kirschallee 1, 53115 Bonn, Germany
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Olaf STRICKER;
Olaf STRICKER
†Botanisches Institut der Universität Bonn, Abteilung Molekulare Biochemie, Kirschallee 1, 53115 Bonn, Germany
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Herbert BÖHME;
Herbert BÖHME
†Botanisches Institut der Universität Bonn, Abteilung Molekulare Biochemie, Kirschallee 1, 53115 Bonn, Germany
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Carlos GÓMEZ-MORENO;
Carlos GÓMEZ-MORENO
*Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009-Zaragoza, Spain
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M. Luisa PELEATO
M. Luisa PELEATO
‡
*Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009-Zaragoza, Spain
‡To whom correpondence should be addressed.
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Publisher: Portland Press Ltd
Received:
November 02 1995
Revision Received:
January 02 1996
Accepted:
January 12 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 316 (1): 157–160.
Article history
Received:
November 02 1995
Revision Received:
January 02 1996
Accepted:
January 12 1996
Citation
Pedro RAZQUIN, Maria F. FILLAT, Stefan SCHMITZ, Olaf STRICKER, Herbert BÖHME, Carlos GÓMEZ-MORENO, M. Luisa PELEATO; Expression of ferredoxin-NADP+ reductase in heterocysts from Anabaena sp. Biochem J 15 May 1996; 316 (1): 157–160. doi: https://doi.org/10.1042/bj3160157
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