1. Electron microscopy of solubilized photosystem II (PSII) complexes and PSII in spinach thylakoid membranes has been carried out and the results have been compared with data obtained from ordered two-dimensional arrays of PSII. Membrane-bound PSII is roughly rectangular (17.6 nm× 14.1 nm) with a central stain cavity surrounded by four major lumenal domains. A comparison between the averaged projections of single (non-ordered) particles at 3.8 nm resolution and the Fourier projection maps obtained from ordered arrays (at 2–3 nm resolution) reveals close similarity and excludes the possibility that PSII observed in two-dimensional ordered arrays represents an unusual subpopulation. 2. After detergent solubilization, PSII adopts various aggregation states which were analysed by electron microscopy in conjunction with single-particle averaging. Two different types of projection of roughly rectangular shape and of dimensions 30 nm×17 nm manifesting themselves as tetrameric sandwich structures have been revealed. This conclusion is supported by the presence of at least two axes of 2-fold rotational symmetry running perpendicular to each other and intersecting at the centre of the oligomer. Comparisons of the structures of detergent-solubilized and native PSII show that the oligomerization of PSII can be artificially induced by the process of membrane solubilization.
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Research Article|
April 15 1996
Structure of photosystem II in spinach thylakoid membranes: comparison of detergent-solubilized and native complexes by electron microscopy
William V. NICHOLSON;
William V. NICHOLSON
‡
*Department of Biochemistry and Applied Molecular Biology, UMIST, P.O. Box 88, Manchester M60 1QD, U.K.
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Fiona H. SHEPHERD;
Fiona H. SHEPHERD
§
†Department of Biochemistry and Molecular Biology and Department of Genetics, University of Leeds, Leeds LS2 9JT, U.K.
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Mark F. ROSENBERG;
Mark F. ROSENBERG
*Department of Biochemistry and Applied Molecular Biology, UMIST, P.O. Box 88, Manchester M60 1QD, U.K.
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Robert C. FORD;
Robert C. FORD
∥
*Department of Biochemistry and Applied Molecular Biology, UMIST, P.O. Box 88, Manchester M60 1QD, U.K.
∥ To whom correspondence should be addressed.
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Andreas HOLZENBURG
Andreas HOLZENBURG
†Department of Biochemistry and Molecular Biology and Department of Genetics, University of Leeds, Leeds LS2 9JT, U.K.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 315 (2): 543–547.
Citation
William V. NICHOLSON, Fiona H. SHEPHERD, Mark F. ROSENBERG, Robert C. FORD, Andreas HOLZENBURG; Structure of photosystem II in spinach thylakoid membranes: comparison of detergent-solubilized and native complexes by electron microscopy. Biochem J 15 April 1996; 315 (2): 543–547. doi: https://doi.org/10.1042/bj3150543
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