The properties of three different β-isoforms of phospholipase C (PLC) were analysed using substrate lipids dispersed in phospholipid vesicles, phospholipid–detergent mixed micelles and phospholipid monolayers spread at an air–water interface. Phosphatidylinositol 4,5-bisphosphate hydrolysis went virtually to completion in monolayers, but inositol trisphosphate production was curtailed prematurely in vesicular and micellar assays. Assays were linear for less than 2 min with vesicles; the linear portion could be significantly extended in micelles by increasing the ratio of micelles to enzyme molecules. However, onset of a second lower rate of substrate hydrolysis always occurred when ⩽ 10% of PtdIns(4,5)P2 had been utilized. This was not due to enzyme inactivation in the micellar interface, determined by addition of fresh substrate or fresh enzyme after the slow phase of activity had started, nor was it due to overt product inhibition of PLC or apparent entrapment of PLC at the micelle surface. These results are similar to those seen in assays using bacterial PLC and we suggest that the biphasic kinetics may be due to product-dependent changes in the presentation of substrate lipid to PLC in lamellar assays, leading to reduced activity.
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Research Article|
March 15 1996
Time-dependent inhibition of phospholipase Cβ-catalysed phosphoinositide hydrolysis: a comparison of different assays
Stephen R. JAMES;
Stephen R. JAMES
‡
*Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee, DD1 4HN, U.K.
‡To whom correspondence should be addressed.
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Sheila SMITH;
Sheila SMITH
*Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee, DD1 4HN, U.K.
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Andrew PATERSON;
Andrew PATERSON
†Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, NC, U.S.A.
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T. Kendall HARDEN;
T. Kendall HARDEN
†Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, NC, U.S.A.
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C. Peter DOWNES
C. Peter DOWNES
*Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee, DD1 4HN, U.K.
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Publisher: Portland Press Ltd
Received:
October 06 1995
Revision Received:
November 10 1995
Accepted:
November 13 1995
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 314 (3): 917–921.
Article history
Received:
October 06 1995
Revision Received:
November 10 1995
Accepted:
November 13 1995
Citation
Stephen R. JAMES, Sheila SMITH, Andrew PATERSON, T. Kendall HARDEN, C. Peter DOWNES; Time-dependent inhibition of phospholipase Cβ-catalysed phosphoinositide hydrolysis: a comparison of different assays. Biochem J 15 March 1996; 314 (3): 917–921. doi: https://doi.org/10.1042/bj3140917
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