We have reported that two inositol 1,4,5-trisphosphate binding proteins, with molecular masses of 85 and 130 kDa, were purified from rat brain; the former protein was found to be the ∆1-isoenzyme of phospholipase C (PLC-∆1) and the latter was an unidentified novel protein [Kanematsu, Takeya, Watanabe, Ozaki, Yoshida, Koga, Iwanaga and Hirata (1992) J. Biol. Chem. 267, 6518-6525]. Here we describe the isolation of the full-length cDNA for the 130 kDa Ins(1,4,5)P3 binding protein, which encodes 1096 amino acids. The predicted sequence of the 130 kDa protein had 38.2% homology to that of PLC-∆1. Three known domains of PLC-∆1 (pleckstrin homology and putative catalytic X and Y domains) were located at residues 110-222, 377-544 and 585-804 with 35.2%, 48.2% and 45.8% homologies respectively. However, the protein showed no PLC activity to phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol. The 130 kDa protein expressed by transfection in COS-1 cells bound Ins(1,4,5)P3 in the same way as the molecule purified from brain. Thus the 130 kDa protein is a novel Ins(1,4,5)P3 binding protein homologous to PLC-∆1, but with no catalytic activity. The functional significance of the 130 kDa protein is discussed.
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January 1996
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Research Article|
January 01 1996
A new inositol 1,4,5-trisphosphate binding protein similar to phospholipase C-δ1
Takashi KANEMATSU;
Takashi KANEMATSU
*Department of Biochemistry, Faculty of Dentistry, Kyushu University, Fukuoka 812-82, Japan
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Yoshio MISUMI;
Yoshio MISUMI
†Department of Biochemistry, Faculty of Medicine, Fukuoka University, Fukuoka 814-80, Japan
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Yutaka WATANABE;
Yutaka WATANABE
‡Department of Applied Chemistry, Faculty of Engineering, Ehime University, Matsuyama 790, Japan
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Shoichiro OZAKI;
Shoichiro OZAKI
‡Department of Applied Chemistry, Faculty of Engineering, Ehime University, Matsuyama 790, Japan
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Toshitaka KOGA;
Toshitaka KOGA
*Department of Biochemistry, Faculty of Dentistry, Kyushu University, Fukuoka 812-82, Japan
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Sadaaki IWANAGA;
Sadaaki IWANAGA
§Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812-81, Japan
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Yukio IKEHARA;
Yukio IKEHARA
†Department of Biochemistry, Faculty of Medicine, Fukuoka University, Fukuoka 814-80, Japan
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Masato HIRATA
Masato HIRATA
¶
*Department of Biochemistry, Faculty of Dentistry, Kyushu University, Fukuoka 812-82, Japan
¶To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
May 30 1995
Revision Received:
August 14 1995
Accepted:
August 30 1995
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 313 (1): 319–325.
Article history
Received:
May 30 1995
Revision Received:
August 14 1995
Accepted:
August 30 1995
Citation
Takashi KANEMATSU, Yoshio MISUMI, Yutaka WATANABE, Shoichiro OZAKI, Toshitaka KOGA, Sadaaki IWANAGA, Yukio IKEHARA, Masato HIRATA; A new inositol 1,4,5-trisphosphate binding protein similar to phospholipase C-δ1. Biochem J 1 January 1996; 313 (1): 319–325. doi: https://doi.org/10.1042/bj3130319
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