The Beginner's Guide series covers key techniques and offers the scientifically literate but not necessarily expert audience a background briefing on the underlying science of a technique that is (or will be) widely used in molecular bioscience. The series covers a mixture of techniques, including some that are well established amongst a subset of our readership but not necessarily familiar to those in different specialisms. This Beginner's Guide introduces solution small-angle X-ray scattering (SAXS). Solution small-angle X-ray scattering (SAXS) is a powerful technique, which is complementary to different bioanalytical and structural methods. With straightforward data collection procedures and minimal restrictions in sample environment, information on size, shape and conformational flexibility of biological macromolecules and complexes in near native solutions can be rapidly obtained. Here, we highlight the recent developments that have advanced SAXS to a versatile tool with the capability to enrich almost any biochemical study.
Skip Nav Destination
Article navigation
Beginner's Guide|
January 31 2020
A beginner's guide to solution small-angle X-ray scattering (SAXS)
Melissa Gräwert;
Melissa Gräwert
1European Molecular Biology Laboratory, Hamburg Unit, Germany
Search for other works by this author on:
Dmitri Svergun
Dmitri Svergun
1European Molecular Biology Laboratory, Hamburg Unit, Germany
Search for other works by this author on:
Publisher: Portland Press Ltd
Online ISSN: 1740-1194
Print ISSN: 0954-982X
2020 © The Authors
2020
Published by Portland Press Limited under the Creative Commons Attribution License 4.0 (CC BY-NC-ND)
Biochem (Lond) (2020) 42 (1): 36–42.
Citation
Melissa Gräwert, Dmitri Svergun; A beginner's guide to solution small-angle X-ray scattering (SAXS). Biochem (Lond) 31 January 2020; 42 (1): 36–42. doi: https://doi.org/10.1042/BIO04201036
Download citation file: